Top Questions

What’s unique about the ProTrap XG?

Protein precipitation is a proven technique and is remarkably effective when performed properly. The challenge is in reproducibility- especially in low microgram protein amounts, when a pellet may not be visible. The ProTrap XG eliminates the possibility that you will accidentally disturb your protein pellet and can improve throughput.

Do you have published research results?

Our Chief Scientist, Alan Doucette, Senior Scientist, Victoria Miller, and Research Associate, Jessica Nickerson along with other members of the Proteoform Scientific R&D team regularly publish posters and papers on the ProTrap XG. You can view these any time in our Science section.

What type of samples can be used in the ProTrap XG?

Protein samples that are clarified and that can be precipitated using acetone have been successfully processed using the ProTrap. Our team is actively working on sample processing protocols to provide users with complex samples input parameters for successful precipitation. Low molecular weight proteins (<5kDa) tend not to precipitate well. We are currently exploring new strategies to successfully process these smaller proteins.

How does the ProTrap XG clean samples?

The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.

General Questions

Do you have an automated solution?

A 96 well-plate version of the ProTrap XG is a part of our product pipeline. Contact us for updates regarding product availability and more.

Do you have published research results?

Our Chief Scientist, Alan Doucette, Senior Scientist, Victoria Miller, and Research Associate, Jessica Nickerson along with other members of the Proteoform Scientific R&D team regularly publish posters and papers on the ProTrap XG. You can view these any time in our Science section.

Do you sell a kit with reagents?

ProTrap XG reagent kits are not available currently but will be coming soon. We’re working on kits for both top-down and bottom-up workflows to launch in 2021. Would you like to get notified when it becomes available? Sign up for updates info@proteoform.com

How does the ProTrap need to be stored?

The ProTrap requires no special storage conditions, whatsoever.

What quantities is the ProTrap XG sold in?

The ProTrap XG is available in packs of 10, 50, or 100, however customized quantities can be made available upon request. Reach out to us at info@proteoform.com to discuss options.

Sample Prep Questions

What type of yield can I expect with the ProTrap XG?

Users typically see greater than 95% recovery of proteins, though your recovery may vary depending on the characteristics of the proteins you are precipitating.

What level of purity can I expect with the ProTrap XG?

The ProTrap XG is capable of removing over 99.8% of a maximum 2% starting sodium dodecyl sulfate (SDS) solution. It can also eliminate or reduce many salts and other buffer components. We are actively researching just what these limits look like for other buffer components, especially detergents.

Does the ProTrap XG do peptide cleanup?

Our team is currently exploring new cleanup strategies in low molecular weight (<5 kDa) proteins and peptides through precipitation.

What’s the volume of sample that the ProTrap XG can handle?

The Filtration Cartridge holds up to 500 microliters. But keep in mind, this volume includes the organic solvent used for precipitation, which is why we suggest up to 100 microliters of sample solution.

How much protein can the ProTrap XG prepare?

Our research team has successfully recovered microgram quantities of protein, down to 5 micrograms. We recommend that you use no more than 200 micrograms of protein using our current protocols. Whatever amount of protein you use, it should be in no more than 100 microlitres.

What type of samples can be used in the ProTrap XG?

Protein samples that are clarified and that can be precipitated using acetone have been successfully processed using the ProTrap. Our team is actively working on sample processing protocols to provide users with complex samples input parameters for successful precipitation. Low molecular weight proteins (<5kDa) tend not to precipitate well. We are currently exploring new strategies to successfully process these smaller proteins.

How does the ProTrap XG clean samples?

The ProTrap XG uses protein precipitation, aggregating the protein in a solvent. Anything soluble in the solvent is washed away when the solvent is removed during a centrifugation step. The precipitated protein is retained by the integrated membrane, while solvent-soluble contaminants are flushed through. Our protocols are based on methods published by our Chief Scientist, Alan Doucette and other researchers in peer-reviewed research articles.

Ordering Questions

How does the ProTrap need to be stored?

The ProTrap requires no special storage conditions, whatsoever.

What are my payment options?

We accept payment via credit card, PayPal, electronic funds transfer or wire transfer.

Do you ship the ProTrap XG worldwide?

Yes, the ProTrap is ready to ship anywhere in the world from our headquarters in Halifax, NS.

How do I order the ProTrap XG?

You can order by email at sales@proteoform.com or by phone. Or if you need a quote we can provide that too.

What quantities is the ProTrap XG sold in?

The ProTrap XG is available in packs of 10, 50, or 100, however customized quantities can be made available upon request. Reach out to us at info@proteoform.com to discuss options.