Mass spec results can be compromised in many ways, PEG is one of those contaminants that can sneak up on you.
As we know, the mass spectrometry data can only reflect the fraction of the sample that gets injected.
Even the best mass spectrometer in the world can only detect the portion of the proteome that is successfully extracted and conserved across the sample prep workflow—so it is critical that the extraction buffer and denaturants that are employed are optimal to recover the entire biological proteome.
Sodium dodecyl sulphate (SDS) also known as sodium lauryl sulfate, is recognized as one of the best detergents for solubilizing protein.
Every proteomicist has their own list of reasons for avoiding solvent-based precipitation. If only we could eliminate these drawbacks.
Look forward to seeing you at 2021 Virtual US HUPO this week on Gather Town. Our Research Associate, Jessica Nickerson will be presenting her latest scientific poster.
Energized for the 2020 SUMS-RAS Mass Spec: The Next Generation on screen today and tomorrow. A great line-up of speakers, opportunities to learn what’s on the mass spec horizon awaits. Watch the poster overview presentation here
This week at HUPO Connect our Senior Scientist, Victoria Miller and team will present our latest research in Poster P150 – Removal of PEG Contaminants From Peptides Through An Off-Line ION-Exchange Spin Column. Check it out if you are attending HUPO Connect 2020 and if not get in touch with Victoria email@example.com for a copy … Continued
You’ve just spent weeks nurturing or gathering your precious protein samples. By your calculations, there should be just enough material to complete your experiments. The workflow begins; an extraction, a purification step, maybe even some trypsin, then off to the mass spectrometry lab. But when the results finally come back, the data are, shall we say, beneath your expectations. But why?
Knowing that precipitation can easily be used for reliable recovery, I also wanted to make it simple. I envisioned a device where protein solution, containing all their impurities, combined with organic solvent, and with the push of a button, purified protein is separated from the solvent. I assumed we could buy some type of device to do just that but quickly realized no such tool had yet been made. So I made one.