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Sample preparation for bottom-up proteomics using a disposable cartridge (ProTrap XG) equipped with chitosan-immobilized trypsin

Subin Rajendran and Alan Doucette, Department of Chemistry, Dalhousie University, Vershuren Centre for Sustainability in Energy and the Environment

Introduction

  • Conventional protein precipitation is slow & erratic as a front-end method for bottom-up proteomics.
  • A two-step cartridge system was developed by our group previously (Crowell et al., 2015) for effective sample prep., facilitating the effective removal of SDS
  • while maintaining high protein recovery.
  • Chitosan matrix with immobilized trypsin is used here, to enhance, automate and facilitate rapid hydrolysis of proteins recovered by ProTrap XGTM.